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The buildings of the new compounds were identified on The idea in their spectral knowledge, which includes 1D and second NMR, HR-MS plus the gene cluster liable for the biosynthesis of phenazines was identified. All phenazine derivatives confirmed antimicrobial exercise in opposition to the Gram-good Bacillus subtilis, though compounds 1–three and five also inhibited progress of the Gram-detrimental Escherichia coli.
It's nevertheless unclear which genes are chargeable for the glycosylation of the endophenazine aglycones, as no this sort of genes have been observed within just or in shut proximity of your epa gene cluster (Desk four). Therefore, the genes answerable for the transfer and methylation with the rhamnose moiety to both the carboxyl or even the hydroxyl group from the phenazines needs to be Situated elsewhere around the chromosome. Analysis to recognize the genes and enzymes liable for the glycosylation in the endaphenazines is at present in progress.
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